- PolyGLYCOPLEX – for complex carbohydrates
- PolyCAT A – for cation-exchange of proteins
- Hydrophilic Interaction Chromatography (HILIC)
- Size Exclusion Chromatography (SEC)
- PolySULFOETHYL Aspartamide – for cation-exchange of peptides
- PolyPROPYL-, PolyETHYL-, PolyMETHYL Aspartamide- for hydrophobic interaction chromatography (HIC) of proteins and peptides
- Getting rid of SDS
- PolyWAX LP – for anion-exchange of proteins
- Scrapie (in sheep)
- Chronic wasting disease (in elk and mule deer)
PolyLC Inc., Columbia USA has developed unique materials and methods
for HPLC of proteins and peptides. The polypeptide-based coatings
permit the resolution of many mixtures not possible with other methods.
PolyLC Inc.’s product line includes columns and cartridges for Hydrophobic
Interaction Chromatography, Hydrophilic Interaction Chromatography, cation
exchange of peptides, selective removal of SDS from peptide digests, anion-exchange
of proteins, and HPLC of complex carbohydrates. PolyLC Inc. can offer
technical support for the most difficult biochemical separations.
PRODUCT RANGE
PolyLC do PRIONS
Recent outbreaks of bovine spongiform encephalopathy (BSE) or mad cow disease in Europe have highlighted the need to develop a method to identify pathogenic prion proteins in the blood of animals and humans.
In collaboration with Dr. Mary Jo Schmerr of the United States Department
of Agriculture (USDA), PolyLC has helped to develop the first practical
test for pathogenic prion proteins in blood. Experimentally, the
test has given positive results in a variety of TSE (Transmissible Spongiform
Encephalopathy) diseases, including:
and similar prion diseases in hamster, llama, chimpanzee and humans
At present the test can detect prion protein in the picogram range
per ml. blood. This is sufficiently sensitive to give positive results
in infected animals before clinical symptoms develop. The test has
not yet been validated. Validation studies are in progress in the case
of scrapie. That will require correlation of the positive results
with the animals subsequently developing clinical cases of disease.
This should take 1-3 more years. In the interim, all use of this
test should be considered to be research rather than definitive diagnosis.
PolyLC’s HPLC columns of PolyHYDROXYETHYL Aspartamide permit the
isolation of pure pathogenic prion protein from extracts from brain and
other tissues of diseased animals. The protein is obtained free of
detergent with recovery >90%, in a form that readily forms complexes with
anti-prion antibodies
For more technical information contact Poly LC at: http://www.chromatechnologies.com
For Pricing, Availability
and Ordering Information Contact: info@specon.com.au
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